Coding

Part:BBa_K5062029:Design

Designed by: Soumik Ojha   Group: iGEM24_HKU-HongKong   (2024-09-27)


SARS-CoV-2-Proofreading Domain (nsP14)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 565
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 565
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 565
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 565
    Illegal AgeI site found at 1157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 416


Design Notes

nsP14 needs to be on the 3' end of the polymerase.


Source

NCBI Database

References